Effects of Silane Monolayers on Lysophosphatidylcholine (LysoPC) Detection by Desorption Ionization on Silicon Mass Spectrometry (DIOS-MS) in Solution and Plasma
Résumé
Desorption ionization on silicon mass spectrometry (DIOS-MS) enables the high throughput analysis of low molecular weight biomolecules. However, the detection of metabolites biomarkers from complex fluids, like plasma, is subordinated to sample pre-treatments which limit clinical applications. Herein, we show that porous silicon chemically modified using monolayers of n-propyldimethylmetoxysilane molecules, can be a good candidate for the fingerprinting of Lysophosphatidylcholine (LysoPC) in plasma, without any pre-treatment of the sample, for DIOS-MS-based diagnosis like sepsis diagnosis. Results are correlated to the location of LysoPC molecules inside/outside the pores, determined by time of flight-secondary ions mass spectrometry profiling, and to their physico-chemical properties.
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