Cell painting transfer increases screening hit rate - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biological Imaging Année : 2023

Cell painting transfer increases screening hit rate

Ethan Cohen
  • Fonction : Auteur
Maxime Corbe
  • Fonction : Auteur
Cláudio Franco
  • Fonction : Auteur
Francisca Vasconcelos
  • Fonction : Auteur
Franck Perez
  • Fonction : Auteur
Elaine del Nery
  • Fonction : Auteur
Guillaume Bollot
  • Fonction : Auteur

Résumé

Abstract Drug discovery uses high throughput screening to identify compounds that interact with a molecular target or that alter a phenotype favorably. The cautious selection of molecules used for such a screening is instrumental and is tightly related to the hit rate. In this work, we wondered if cell painting, a general-purpose image-based assay, could be used as an efficient proxy for compound selection, thus increasing the success rate of a specific assay. To this end, we considered cell painting images with 30,000 molecules treatments, and selected compounds that produced a visual effect close to the positive control of an assay, by using the Frechet Inception Distance. We then compared the hit rates of such a preselection with what was actually obtained in real screening campaigns. As a result, cell painting would have permitted a significant increase in the success rate and, even for one of the assays, would have allowed to reach 80% of the hits with 10 times fewer compounds to test. We conclude that images of a cell painting assay can be directly used for compound selection prior to screening, and we provide a simple quantitative approach in order to do so.

Dates et versions

hal-04081469 , version 1 (25-04-2023)

Identifiants

Citer

Ethan Cohen, Maxime Corbe, Cláudio Franco, Francisca Vasconcelos, Franck Perez, et al.. Cell painting transfer increases screening hit rate. Biological Imaging, 2023, 3, pp.e4. ⟨10.1017/S2633903X23000077⟩. ⟨hal-04081469⟩

Collections

ENS-PARIS CNRS PSL
2 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More