Biodegradation of cellulose and xylan by a paddy pest, Oxya chinensis - Archive ouverte HAL
Article Dans Une Revue ANNALS OF BIOLOGICAL RESEARCH Année : 2010

Biodegradation of cellulose and xylan by a paddy pest, Oxya chinensis

Résumé

Oxya chinensis Thunberg is a very serious paddy pest in Pakistan and is a major cause of rice crop deterioration. Total cellular protein extract was able to degrade plant biomass from sugar cane bagasse, as observed under microscope. Its endoglucanase and xylanase activities were characterized. The endoglucanase activity showed two pH optima 2.0 and 7.0 at 50-60°C. Endobeta-1,4-glucanase activity enhanced by calcium ions. A reducing agent beta-mercaptoethanol inhibited the enzyme activity, showing the involvement in stabilizing the three-dimensional structure of the molecule. The enzyme product was identified as glucose by thin layer chromatography. For xylanase the pH optimum was 4.2 and 7.0-8.0. CaCl 2 and 2mercaptoethanol are proven to be activator in lower concentration for xylanase activity.
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hal-04051711 , version 1 (30-03-2023)

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  • HAL Id : hal-04051711 , version 1

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Amtul Jamil Sami, Farhana Tabassum, Shakoori A. R.. Biodegradation of cellulose and xylan by a paddy pest, Oxya chinensis. ANNALS OF BIOLOGICAL RESEARCH, 2010, 1 (3), pp.01-12. ⟨hal-04051711⟩
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