Biodegradation of cellulose and xylan by a paddy pest, Oxya chinensis
Résumé
Oxya chinensis Thunberg is a very serious paddy pest in Pakistan and is a major cause of rice crop deterioration. Total cellular protein extract was able to degrade plant biomass from sugar cane bagasse, as observed under microscope. Its endoglucanase and xylanase activities were characterized. The endoglucanase activity showed two pH optima 2.0 and 7.0 at 50-60°C. Endobeta-1,4-glucanase activity enhanced by calcium ions. A reducing agent beta-mercaptoethanol inhibited the enzyme activity, showing the involvement in stabilizing the three-dimensional structure of the molecule. The enzyme product was identified as glucose by thin layer chromatography. For xylanase the pH optimum was 4.2 and 7.0-8.0. CaCl 2 and 2mercaptoethanol are proven to be activator in lower concentration for xylanase activity.
Domaines
Sciences du Vivant [q-bio]
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biodegradation-of-cellulose-and-xylan-by-a-paddy-pest-oxya-chinensis.pdf (481.83 Ko)
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