MYO10 promotes transzonal projection-dependent germ line-somatic contact during mammalian folliculogenesis - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biology of Reproduction Année : 2022

MYO10 promotes transzonal projection-dependent germ line-somatic contact during mammalian folliculogenesis

Sofia Granados-Aparici
  • Fonction : Auteur
Alexander Volodarsky-Perel
  • Fonction : Auteur
Qin Yang
  • Fonction : Auteur
Sibat Anam
  • Fonction : Auteur
Togas Tulandi
  • Fonction : Auteur
William Buckett
  • Fonction : Auteur
Weon-Young Son
  • Fonction : Auteur
Jin-Tae Chung
  • Fonction : Auteur
Shaoguang Jin
  • Fonction : Auteur
Hugh Clarke
  • Fonction : Auteur

Résumé

Abstract Granulosa cells of growing ovarian follicles elaborate filopodia-like structures termed transzonal projections (TZPs) that supply the enclosed oocyte with factors essential for its development. Little is known, however, of the mechanisms underlying the generation of TZPs. We show in mouse and human that filopodia, defined by an actin backbone, emerge from granulosa cells in early stage primary follicles and that actin-rich TZPs become detectable as soon as a space corresponding to the zona pellucida appears. mRNA encoding Myosin10 (MYO10), a motor protein that accumulates at the base and tips of filopodia and has been implicated in their initiation and elongation, is present in granulosa cells and oocytes of growing follicles. MYO10 protein accumulates in foci located mainly between the oocyte and innermost layer of granulosa cells, where it colocalizes with actin. In both mouse and human, the number of MYO10 foci increases as oocytes grow, corresponding to the increase in the number of actin-TZPs. RNAi-mediated depletion of MYO10 in cultured mouse granulosa cell-oocyte complexes is associated with a 52% reduction in the number of MYO10 foci and a 28% reduction in the number of actin-TZPs. Moreover, incubation of cumulus-oocyte complexes in the presence of epidermal growth factor, which triggers a 93% reduction in the number of actin-TZPs, is associated with a 55% reduction in the number of MYO10 foci. These results suggest that granulosa cells possess an ability to elaborate filopodia, which when directed toward the oocyte become actin-TZPs, and that MYO10 increases the efficiency of formation or maintenance of actin-TZPs.

Dates et versions

hal-04037836 , version 1 (20-03-2023)

Identifiants

Citer

Sofia Granados-Aparici, Alexander Volodarsky-Perel, Qin Yang, Sibat Anam, Togas Tulandi, et al.. MYO10 promotes transzonal projection-dependent germ line-somatic contact during mammalian folliculogenesis. Biology of Reproduction, 2022, 107 (2), pp.474-487. ⟨10.1093/biolre/ioac078⟩. ⟨hal-04037836⟩
12 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More