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Article Dans Une Revue PLoS ONE Année : 2020

Toward a better definition of hematopoietic progenitors suitable for B cell differentiation

Résumé

The success of inducing human pluripotent stem cells (hIPSC) offers new opportunities for cell-based therapy. Since B cells exert roles as effector and as regulator of immune responses in different clinical settings, we were interested in generating B cells from hIPSC. We differentiated human embryonic stem cells (hESC) and hIPSC into B cells onto OP9 and MS-5 stromal cells successively. We overcame issues in generating CD34 + CD43 + hematopoietic progenitors with appropriate cytokine conditions and emphasized the difficulties to generate proper hematopoietic progenitors. We highlight CD31 int CD45 int phenotype as a possible marker of hematopoietic progenitors suitable for B cell differentiation. Defining precisely proper lymphoid progenitors will improve the study of their lineage commitment and the signals needed during the in vitro process.
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Dates et versions

hal-04037174 , version 1 (20-03-2023)

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Florian Dubois, Anne Gaignerie, Léa Flippe, Jean-Marie Heslan, Laurent Tesson, et al.. Toward a better definition of hematopoietic progenitors suitable for B cell differentiation. PLoS ONE, 2020, 15 (12), pp.e0243769. ⟨10.1371/journal.pone.0243769⟩. ⟨hal-04037174⟩
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