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Article Dans Une Revue Journal of Bioanalysis & Biomedicine Année : 2018

Effect of Magnesium Sulfate on Mitochondrial Oxygen Consumption Rate In vitro Study

Résumé

Hypoxic insults initiate a cascade of biochemical events that result in irreversible neuronal damage. Magnesium sulfate agent has a possible neuroprotective effect as it can work at different stages of hypoxic brain injury. Objectives: Magnesium sulfate is used in the neonatal management of HIE as an adjunct medication to reduce Hypoxic Neuronal injury. In this Vitro study, we aimed to examine the efficacy of using magnesium sulfate in human cells to reduce oxygen consumption. We used in-vitro method utilizing foreskin as human cell surrogate. Method: Foreskin specimens from healthy newborns were collected immediately after circumcision and processed within 1 h for measuring the cellular rate of O 2 consumption. Samples were placed in the oxygen phosphorescence analyzer and allowed to run for approximately 1 h. Injection then added magnesium sulfate at different doses into the vial. We analyze the O 2 consumption in the samples at different levels of Magnesium Sulfate; the results were plotted using Kaleida Graph TM software. Results: The rate of respiration reduced with increasing the dose of MgSo4. The Cumulative analysis of cellular respiration rate was before and after an addition of MgSO 4. Collectively yielding a (k) value of 0.08 μM O 2 /min µM O 2 min-1 mg-and 0.04 M O 2 /min µM O 2 min-1 mg-respectively with a significant P-value of <0.001. Conclusion: Magnesium sulfate reduces the rate of O 2 Consumption in a dose-dependent manner.
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Dates et versions

hal-04034143 , version 1 (17-03-2023)

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Amna Al Zaabi, Aiman Al Rahmani. Effect of Magnesium Sulfate on Mitochondrial Oxygen Consumption Rate In vitro Study. Journal of Bioanalysis & Biomedicine, 2018, 10, ⟨10.4172/1948-593x.1000206⟩. ⟨hal-04034143⟩
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