Cluster-Assembled Zirconia Substrates Accelerate the Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Nanomaterials Année : 2023

Cluster-Assembled Zirconia Substrates Accelerate the Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells

Sara Castiglioni
Laura Locatelli
Alessandra Cazzaniga
Francesca Maria Orecchio
Tommaso Santaniello
  • Fonction : Auteur
Claudio Piazzoni
  • Fonction : Auteur
Francesca Borghi
Paolo Milani
Jeanette Maier
  • Fonction : Auteur

Résumé

Due to their high mechanical strength and good biocompatibility, nanostructured zirconia surfaces (ns-ZrOx) are widely used for bio-applications. Through supersonic cluster beam deposition, we produced ZrOx films with controllable roughness at the nanoscale, mimicking the morphological and topographical properties of the extracellular matrix. We show that a 20 nm ns-ZrOx surface accelerates the osteogenic differentiation of human bone marrow-derived MSCs (bMSCs) by increasing the deposition of calcium in the extracellular matrix and upregulating some osteogenic differentiation markers. bMSCs seeded on 20 nm ns-ZrOx show randomly oriented actin fibers, changes in nuclear morphology, and a reduction in mitochondrial transmembrane potential when compared to the cells cultured on flat zirconia (flat-ZrO2) substrates and glass coverslips used as controls. Additionally, an increase in ROS, known to promote osteogenesis, was detected after 24 h of culture on 20 nm ns-ZrOx. All the modifications induced by the ns-ZrOx surface are rescued after the first hours of culture. We propose that ns-ZrOx-induced cytoskeletal remodeling transmits signals generated by the extracellular environment to the nucleus, with the consequent modulation of the expression of genes controlling cell fate.

Dates et versions

hal-04015643 , version 1 (06-03-2023)

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Citer

Sara Castiglioni, Laura Locatelli, Alessandra Cazzaniga, Francesca Maria Orecchio, Tommaso Santaniello, et al.. Cluster-Assembled Zirconia Substrates Accelerate the Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells. Nanomaterials, 2023, 13 (5), pp.801. ⟨10.3390/nano13050801⟩. ⟨hal-04015643⟩

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