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Article Dans Une Revue IEEE Journal of Electromagnetics, RF and Microwaves in Medicine and Biology Année : 2023

Millimeter-Wave Pulsed Heating in Vitro: Effect of Pulse Duration

Résumé

The aim of this work is to compare the response of A375 melanoma cells following 90 min of exposure to trains of 1.5 or 6 s millimeter-waves (MMW)-induced thermal pulses with the same temperature dynamics. Phosphorylation of heat shock protein 27 (HSP27) and activation of cleaved Caspase-3 were used as markers of cellular stress and apoptosis, respectively. Immunofluorescence was used to observe and precisely quantify the cellular response as a function of the spatial distribution within the exposed area. Results show that cellular response was stronger when cells were exposed to a train of 1.5 s compared to 6 s heat pulses despite the same average temperature dynamics. Cellular apoptosis induced by 1.5 s pulses was about 50% greater compared to the one induced by 6 s pulses in the area of maximal thermal stress. Similarly, HSP27 phosphorylation was approximately 20% stronger than the one induced by 6 s pulses, and mainly focused within a small area of a few mm(2). Cellular response to MMW induced by pulsed heating does not only depend on the peak, average, and minimum temperature. It is a function of combination of the pulse parameters, including duration, peak power, and period. MMW-induced heat pulses can be efficiently used to induce cellular stress and apoptosis in melanoma cells as a promising innovative tool for the treatment of superficial skin cancer. Adaptative therapies might be envisaged by tuning the pulse shape as a function of the desired effect.
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Dates et versions

hal-04009668 , version 1 (14-03-2023)

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Paternité - Pas d'utilisation commerciale

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Citer

Rosa Orlacchio, Yann Le Page, Yves Le Dréan, Maxim Zhadobov. Millimeter-Wave Pulsed Heating in Vitro: Effect of Pulse Duration. IEEE Journal of Electromagnetics, RF and Microwaves in Medicine and Biology, 2023, pp.1-8. ⟨10.1109/JERM.2022.3229738⟩. ⟨hal-04009668⟩
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