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Article Dans Une Revue Electrophoresis Année : 2023

Online large volume sample staking preconcentration and separation of enantiomeric GHRH analogs by capillary electrophoresis

Résumé

A capillary electrophoresis method is proposed to analyze the four most wellknown growth hormone–releasing hormone (GHRH) analogs that are misused by athletes.Dimethyl-β-cyclodextrin used as a chiral selector allowed, for the first time, the separation of those basic peptide analogs, including enantiopeptides (sermorelin and CJC-1293) that differ by the chirality of only one amino acid. To increase the method sensitivity, electrokinetic preconcentration methods have been investigated. The large volume sample stacking with polarity switching (PS-LVSS) method with an injected sample volume corresponding to 80% of the capillary one was found superior to the sweeping in terms of signal enhancement factor (SEF). Acid and organic solvent addition to the sample (0.1 mM phosphoric acid with 30%methanol) led to a twofold signal improvement, when compared to water as a matrix. We increased capillary dimensions to provide a signal enhancement through the injection of a larger sample volume. Finally, using a combination of the optimized PS-LVSS preconcentration with the chiral capillary zone electrophoresis (CZE), the GHRH analogs were separated and limits of detection between 75 and 200 ng/mL were reached. This method was successfully applied to urine after a desalting step. An optimized C18 SPE was used for that purpose in order to provide low sample conductivity (<130 μS/cm) and preserve the efficiency of LVSS preconcentration. SEF of 640 was obtained with desalted urine spiked with sermorelin by comparison to the CZE (without preconcentration) method.
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Dates et versions

hal-04008206 , version 1 (28-02-2023)

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Joanie Otin, N Thuy Tran, Aurélie Benoit, Corinne Buisson, Myriam Taverna. Online large volume sample staking preconcentration and separation of enantiomeric GHRH analogs by capillary electrophoresis. Electrophoresis, In press, ⟨10.1002/elps.202200278⟩. ⟨hal-04008206⟩
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