BACKGROUND: A new morphometric fixed-bearing unicompartmental knee arthroplasty (UKA) system has been introduced to address the anatomical patient-specific challenges. It was our hypothesis that accurate restoration of the patient-specific anatomy would restore normal knee kinematics after UKA. Therefore, we aimed in this cadaveric study to analyze the impact of a medial morphometric UKA on (1) the varus-valgus and anterior-posterior stability of the knee, (2) the knee kinematics during standardized activities of the daily living, and (3) the patellar tracking, measured using a dedicated robotic testing protocol.METHODS: Eight human knee specimens underwent full-leg computed tomography CT scanning and comprehensive robotic assessments of tibiofemoral and patellofemoral kinematics. Specimens were tested in the intact state and after implantation of a fixed-bearing medial UKA. Assessments included passive flexion, laxity testing and simulations of level walking, lunge, and stair descent.RESULTS: Medial and lateral joint laxity after UKA closely resembled intact laxity across the full arc of flexion. Anterior-posterior envelope of motion showed a close match between the intact and UKA groups. Net rollback and average laxity were both not statistically different. Simulation of activities of daily living showed a close match in the anterior-posterior motion profile between the medial condyle and lateral condyle. Patellar tilt and medial-lateral shift during knee flexion matched closely between groups.CONCLUSION: Functional assessment of this UKA system shows nearly identical behavior to the intact knee. Fixed-bearing UKA with morphometric, compartment-specific geometry and precise mechanical instrumentation replicates complex knee balance and kinematics.