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Article Dans Une Revue European Journal of Endocrinology Année : 2006

Adiponutrin gene is regulated by insulin and glucose in human adipose tissue

Geneviève Beauregard
  • Fonction : Auteur
May Faraj
  • Fonction : Auteur
Noël Peretti
  • Fonction : Auteur
Pierre-Henri Ducluzeau
  • Fonction : Auteur
Martine Laville
  • Fonction : Auteur
Rémi Rabasa-Lhoret
  • Fonction : Auteur
Hubert Vidal
  • Fonction : Auteur
Karine Clément
  • Fonction : Auteur

Résumé

Objective : Adiponutrin is a new transmembrane protein specifically expressed in adipose tissue. In obese subjects, short- or long-term calorie restriction diets were associated with a reduction in adiponutrin gene expression. Adiponut.rin mRNA level was previously shown to be negatively correlated with fasting glucose plasma levels and associated with insulin sensitivity of non-diabetic obese and non-obese subjects. The purpose of the present work was to get more insight into the regulation of adiponutrin gene expression by insulin and/or glucose using clamp studies and to examine its potential dysregulation in subjects with a deterioration of glucose homeostasis. Methods : Adiponutrin gene expression was quantified by reverse transcriptase-quantitative PCR in s.c. adipose tissue of healthy lean subjects after an euglycemic hyperinsulinemic clamp (EGHI), a hyperglycemic euinsulinemic clamp, and a hyperglycemic hyperinsulinemic (HGHI) clamp. Adiponutrin gene expression was also analyzed in patients with different levels of insulin resistance. Results : During EGHI, insulin infusion induced adiponutrin gene expression 8.4-fold ( P = 0.008). Its expression was also induced by glucose infusion, although to a lesser extend (2.2-fold, P = 0.03). Infusion of both insulin and glucose (HGHI) had an additive effect on the adiponutrin expression (tenfold, P = 0.008). In a pathological context, adiponutrin gene was highly expressed in the adipose tissue of type-1 diabetic patients with chronic hyperglycemia compared with healthy subjects. Conversely, adiponutrin gene expression was significantly reduced in type-2 diabetics ( P = 0.01), but remained moderately regulated in these patients after the EGHI clamp (2.5-fold increased). Conclusion : These results suggest a strong relationship between adiponutrin expression, insulin sensitivity, and glucose metabolism in human adipose tissue.

Dates et versions

hal-04000862 , version 1 (22-02-2023)

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Citer

Marthe Moldes, Geneviève Beauregard, May Faraj, Noël Peretti, Pierre-Henri Ducluzeau, et al.. Adiponutrin gene is regulated by insulin and glucose in human adipose tissue. European Journal of Endocrinology, 2006, 155 (3), pp.461-468. ⟨10.1530/eje.1.02229⟩. ⟨hal-04000862⟩
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