In order to maintain their survival, species often make trade-offs between survival and reproduction, often resulting in reduced reproduction. For instance, in temperate areas Drosophila species usually reduce or even stop completely their reproductive activity to cope with cold stressful winter period, in a process called reproductive dormancy. In Drosophila melanogaster, this response is rather weak, reversible and often resumes as soon as temper- atures allow egg maturation (i.e. reproductive quiescence). The responses to overwintering by oogenesis stop suggests a cost of reproduction and/or an increased stress tolerance by reduced reproductive activity; yet, this hypothesis has not been properly tested for cold tolerance in Drosophila. Here, we compared cold tolerance of gravid versus virgin females, assuming that the latter would lay fewer eggs. We performed a range of cold tolerance assays to determine if virginity promoted cold tolerance. Virgin females indeed laid fewer eggs than gravid ones, but this quantitative reduction of oogenesis did not promote cold stress tolerance. We then attempted to artificially induce a complete stop of oviposition by pharmacological interventions. To do so, we used Rapamycin and Phloroglucinol, two compounds known to reduce the oviposition’s activity in Drosophila species. Our results showed that Rapamycin’s effect on fecundity was mixed and that it did not enhance the flies’ cold tolerance. On the other hand, Phloroglucinol suppressed oviposition activity and significantly enhanced cold tolerance. Further works is ongoing to clarify the effect of Phloroglucinol on stress tolerance.