The importance of fluid-elastic coupling forces in tube bundle vibrations is well documented and can hardly be over-emphasized, in view of their damaging potential. Even when adequate tube supports are provided to suppress fluid-elastic instabilities, the flow-coupling forces still affect the dynamical tube responses and remain a significant issue, in particular concerning the vibro-impact motions of tubes assembled using clearance supports. Therefore, the need remains for more advanced models of fluid-elastic coupling, as well as for experimental flow-coupling coefficients to feed and validate such models. In this work, we report an extensive series of experiments performed at CEA-Saclay leading to the identification of stiffness and damping fluid-elastic coefficients, for a 3×5 square tube bundle (D 30 mm, P/D 1.5) subjected to single-phase transverse flow, in a water test-loop. The bundle is rigid, except for the central tube which is mounted on a flexible suspension (two parallel steel blades) allowing for translation motions of the tube in the lift direction. The system is thus single-degree of freedom, allowing fluid-elastic instability to arise through a negative damping mechanism. The flow-coupling stiffness and damping coefficients, Kf(Vr) and Cf(Vr), are experimentally identified as functions of the reduced velocity Vr. The maximum value of the Reynolds number ranged from 105 to 2.16 105 (based on the maximum pitch velocity), according to the tested configuration. Identification is achieved on the basis of changes in tube vibration frequency and reduced damping as a function of flow velocity, assuming a constant fluid added mass. In the present experiments, coefficient identification is performed well beyond the instability boundary, by using active control, thereafter allowing exploration of a significant range of flow velocity. The modal frequency and the modal mass of the system are respectively modified by changing the tube suspension stiffness, and/or by adding a mass to the system. We can thus assert how the fluid-elastic coefficients change, for this configuration, with these two system parameters, all other parameters being kept constant. The results obtained from the configurations tested suggest that formulations for coefficient reduction may be improved, in order to better collapse the identified data.