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Communication Dans Un Congrès Année : 2022

Metabolomics insight in the response and tolerance of periphytic biofilms to wastewater effluent

Résumé

Recent evidences highlighted the effect of urban effluent on the structure/functions of dowstream periphytic communities associated to increased tolerance to chemical stress. Nevertheless, the molecular mechanisms of these responses remain poorly understood while usual descriptors provide a partial picture of the phenotype of the communities. In this context, this study aims to gain knowledge about the molecular/biochemical responses of periphyton under urban stress through the implementation of untargeted metabolomics approach. To do so, following exposure in indoor channels connected to an urban effluent, the metabolomic responses of the biofilms were characterized in parallel of structural and functional responses. First, no marked effects were noted on the photosynthesis, respiration, primary/secondary production, as well as on biomass whereas exposure to the raw effluent led to tolerance acquisition to further chemical stress. Then, the metabolomic profiles showed discrepancies between all the conditions demonstrating a clear effect of the effluent on the molecular phenotype of the biofilms. Moreover, the clear separation between raw and ultrafiltrated conditions highlighted the potential contribution of the microbes from the effluent in the response. Such pattern paralleled with the shift in microbial diversity. The influence of the microbes was further confirmed through HCA showing that control and ultrafiltrated conditions were clustered together. Further trend analysis revealed up/down regulation on several pathways. Finally, statistical comparison between the metabolomic profiles of raw vs ultrafiltrated conditions highlighted that only 11% down- and 13% up-regulated signals contributed to the tolerance of the biofilm. Overall, this study shows that the metabolomic response is more sensitive than usual descriptors through its ability to discriminate all the experimental conditions. Moreover, it shows that urban microbes contribute to these various molecular/biochemical responses. Further investigations are ongoing to confirm the identity of the candidate metabolites and pathways and discriminate their link to urban microbes vs chemicals.
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Dates et versions

hal-03990145 , version 1 (15-02-2023)

Identifiants

  • HAL Id : hal-03990145 , version 1

Citer

Nicolas Creusot, Louis Carles, Mélissa Eon, Simon Wullschleger, Soizic Morin, et al.. Metabolomics insight in the response and tolerance of periphytic biofilms to wastewater effluent. 7th Biofilm Workshop, Jun 2022, Bordeaux, France. ⟨hal-03990145⟩
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