Mechanisms of Checkpoint Kinase Rad53 Inactivation after a Double-Strand Break in Saccharomyces cerevisiae - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Molecular and Cellular Biology Année : 2007

Mechanisms of Checkpoint Kinase Rad53 Inactivation after a Double-Strand Break in Saccharomyces cerevisiae

Résumé

ABSTRACT In Saccharomyces cerevisiae , double-strand breaks (DSBs) activate DNA checkpoint pathways that trigger several responses including a strong G 2 /M arrest. We have previously provided evidence that the phosphatases Ptc2 and Ptc3 of the protein phosphatase 2C type are required for DNA checkpoint inactivation after a DSB and probably dephosphorylate the checkpoint kinase Rad53. In this article we have investigated further the interactions between Ptc2 and Rad53. We showed that forkhead-associated domain 1 (FHA1) of Rad53 interacts with a specific threonine of Ptc2, T376, located outside its catalytic domain in a TXXD motif which constitutes an optimal FHA1 binding sequence in vitro. Mutating T376 abolishes Ptc2 interaction with the Rad53 FHA1 domain and results in adaptation and recovery defects following a DSB. We found that Ckb1 and Ckb2, the regulatory subunits of the protein kinase CK2, are necessary for the in vivo interaction between Ptc2 and the Rad53 FHA1 domain, that Ckb1 binds Ptc2 in vitro and that ckb1 Δ and ckb2 Δ mutants are defective in adaptation and recovery after a DSB. Our data thus strongly suggest that CK2 is the kinase responsible for the in vivo phosphorylation of Ptc2 T376.

Dates et versions

hal-03990108 , version 1 (15-02-2023)

Identifiants

Citer

Ghislaine Guillemain, Emilie Ma, Sarah Mauger, Simona Miron, Robert Thai, et al.. Mechanisms of Checkpoint Kinase Rad53 Inactivation after a Double-Strand Break in Saccharomyces cerevisiae. Molecular and Cellular Biology, 2007, 27 (9), pp.3378-3389. ⟨10.1128/MCB.00863-06⟩. ⟨hal-03990108⟩

Collections

CEA
3 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More