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Poster De Conférence Année : 2022

Structural Dynamics of biofilms exposed to contaminants and effects on river functions.

Résumé

During the last decades, intensified human activities and emerging contaminants have been disturbing the quality of freshwaters. Such hazardous compounds threat and alter the physical and chemical properties of aquatic ecosystems and consequently the global biodiversity, biological processes and functional services provided by rivers. Assessing quality of freshwaters is thus became of high importance. In that context, bioindication evolves into an indispensable tool for water quality assessment. However, current biological monitoring relies mainly on techniques which need highly-qualified personnel and are time-consuming as well as costly. It includes for example capture-based surveys and observations for vertebrates and invertebrates, or microscopic observation and plate-counting for microorganisms. The use of environmental genomic approaches would be a good solution to develop a rapid, efficient and cost-effective method due to the development of high-throughput sequencing and bioinformatics tools. The aim of our study is to define biofilm-based indicator(s) to assess metal impacts on rivers. River biofilms represent a highly diversified biota (bacteria, algae, fungi and meiofauna) and have the interesting feature of responding quickly and in a sensitive manner to changes in water quality. Contaminants could have repercussions on biological organization of these biofilms. Because they are at the bottom of the autotrophic food chain, impacting these microecosystems would affect biogeochemical cycles of major elements in the river and river functions. To that end, glass slides were exposed to increasing concentrations of cobalt (3 µg/L, 30 µg/L and 60 µg/L) in outdoor mesocosms for 28 days. Growing biofilms were collected by scraping the slides after 7 d, 14 d, 21 d and 28 d of exposure. Cobalt bioaccumulation in biofilms will be determined and its potential impacts on their biological organization will be assessed through molecular approaches. Biofilms are currently analyzed at the level of functional genes related to C, N and S cycles. Bacteria and algae abundance is also assessed using qPCR. Future analyses will examine microorganism diversity as well as their interactions. The overall results will bring insights on the evaluation of metal bioavailability and toxicity towards aquatic communities, and are expected to go deeper into the genomic-based tools improvement in environmental risk assessment.
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Dates et versions

hal-03969004 , version 1 (02-02-2023)

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  • HAL Id : hal-03969004 , version 1

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Sarah Gourgues, Marisol Goñi, Patrick Baldoni-Andrey, Amiel Boullemant, Clémentine Gelber, et al.. Structural Dynamics of biofilms exposed to contaminants and effects on river functions.. SETAC EUROPE 32TH ANNUAL MEETING, May 2022, Copenhagen, Denmark, Denmark. ⟨hal-03969004⟩
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