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Article Dans Une Revue Lab on a Chip Année : 2023

Magnetically localized and wash-free fluorescence immunoassay (MLFIA): proof of concept and clinical applications

O. Ramel
  • Fonction : Auteur
P. Bigotte
  • Fonction : Auteur
P. Kauffmann
  • Fonction : Auteur
D. Kirk
V. Masse
  • Fonction : Auteur
Nora Dempsey
T. Devillers
M. Weidenhaupt

Résumé

Immunoassays are used for many applications in various markets, from clinical diagnostics to the food industry, generally relying on gold-standard ELISAs that are sensitive, robust, and cheap but also timeconsuming and labour intensive. As an alternative, we propose here the magnetically localized and washfree fluorescence immunoassay (MLFIA): a no-wash assay to directly measure a biomolecule concentration, without mixing nor washing steps. To do so, a fluorescence no-wash measurement is performed to generate a detectable signal. It consists of a differential measurement between the fluorescence of fluorophores bound to magnetic nanoparticles specifically captured by micro-magnets against the residual background fluorescence of unbound fluorophores. Targeted biomolecules (antibodies or antigens) are locally concentrated on micro-magnet lines, with the number of captured biomolecules quantitatively measured without any washing step. The performance of the MLFIA platform is assessed and its use is demonstrated with several biological models as well as clinical blood samples for HIV, HCV and HBV detection, with benchmarking to standard analyzers of healthcare laboratories. Thus, we demonstrated for the first time the versatility of the innovative MLFIA platform. We highlighted promising performances with the successful quantitative detection of various targets (antigens and antibodies), in different biological samples (serum and plasma), for different clinical tests (HCV, HBV, HIV).
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Dates et versions

hal-03967137 , version 1 (01-02-2023)

Identifiants

Citer

S. Delshadi, M. Fratzl, O. Ramel, P. Bigotte, P. Kauffmann, et al.. Magnetically localized and wash-free fluorescence immunoassay (MLFIA): proof of concept and clinical applications. Lab on a Chip, 2023, 23 (4), pp.645-658. ⟨10.1039/d2lc00926a⟩. ⟨hal-03967137⟩
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