Solution behavior and encapsulation properties of fatty acid-elastin-like polypeptide conjugates - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue RSC Advances Année : 2023

Solution behavior and encapsulation properties of fatty acid-elastin-like polypeptide conjugates

Résumé

Developing new biomaterials is an active research area owing to their applications in regenerative medicine, tissue engineering and drug delivery. Elastin-like polypeptides (ELPs) are good candidates for these applications because they are biosourced, biocompatible and biodegradable. With the aim of developing ELP-based micelles for drug delivery applications we have synthesized 15 acyl-ELP compounds by conjugating myristic, palmitic, stearic, oleic or linoleic acid to the N-terminus of three ELPs differing in molar mass. The ELP-fatty acid conjugates have interesting solution behavior. They form micelles at low temperatures and aggregate above the cloud point temperature (Tcp). The critical micelle concentration depends on the fatty acid nature while the micelle size is mainly determined by the ELP block length. We were able to show that ELPs were better hydrated in the micelles than in their individual state in solution. The micelles are stable in phosphate-buffer saline at temperatures below the Tcp, which can vary between 20 °C and 38 °C depending on the length or hydrophilicity of the ELP. Acyl-ELP micelles were loaded with the small hydrophobic molecule Nile red. The encapsulation efficiency and release kinetics showed that the best loading conditions were achieved with the largest ELP conjugated to stearic acid.
Fichier principal
Vignette du fichier
Tingting_Zhang_et_al_RSC_Adv_2023_13_2190_with_ESI.pdf (1.68 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03966126 , version 1 (31-01-2023)

Licence

Paternité - Pas d'utilisation commerciale - Partage selon les Conditions Initiales

Identifiants

Citer

Tingting Zhang, Frédéric Peruch, Amélie Weber, Katell Bathany, Martin Fauquignon, et al.. Solution behavior and encapsulation properties of fatty acid-elastin-like polypeptide conjugates. RSC Advances, 2023, 13 (3), pp.2190. ⟨10.1039/d2ra06603c⟩. ⟨hal-03966126⟩

Collections

CNRS INC-CNRS LCPO
49 Consultations
45 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More