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Poster De Conférence Année : 2022

Accumulation and Effects of Cobalt in River Biofilms

Résumé

Cobalt (Co) is an essential trace element involved in several biomolecules. Over the last decade, industrial demand has led to an exponential increase of its extraction. Indeed, Co is intensively used in the energy storage technologies, such as in Co-lithium batteries found in electrical appliances and cars. An increase in dissolved Co concentrations is thus observed in aquatic environments, which might lead to negative impacts on living organisms such as river biofilms. These biofilms, at the base of the trophic network, are composed of bacteria, microalgae, fungi and microfauna. However, little is still known about Co accumulation and toxicity towards biofilms, which prevents adequate protection of freshwater environments. The objective of this study is to better understand the short- and long-term effects of Co on microalgal communities. To this end, mature biofilms were exposed to increasing concentrations of Co (0, 10-7, 10-6, 5.10-6 and 10-5 M) for 7 days using outdoor microcosms (15 L) filled with the Gave de Pau water. Every day the exposure medium was sampled to analyse the concentrations of metals, cations, anions and dissolved organic carbon. Biofilms were also collected after 1, 3 and 7 days of exposure and were examined for different biological parameters. Firstly, the total and intracellular Co concentrations in biofilms were measured after an EDTA wash and acid digestion. This EDTA step allowed us distinguishing between total and intracellular content. The effect of Co will be evaluate at the subcellular levels using untargeted metabolomic analysis and the chlorophyll-a content while shift in microalgal community will be assessed using metabarcoding. In view of the first results obtained concerning the accumulation of Co by biofilms, bioaccumulated Co was mostly intracellular. Very good correlations between dissolved Co concentrations in the exposure medium and intracellular concentrations at each exposure time are observed (R2adj of 0.91, 0.95 and 0.97 respectively after 1, 3 and 7 days of exposure). The analysis of the various biological parameters could allow the characterisation of potential new biomarkers of exposure and the identification of bioindicator species within the exposed biofilms.
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Dates et versions

hal-03960637 , version 1 (27-01-2023)

Identifiants

  • HAL Id : hal-03960637 , version 1

Citer

Simon Colas, B Marie, Mathieu Milhe-Poutingon, Patrick Baldoni-Andrey, Amiel Boullemant, et al.. Accumulation and Effects of Cobalt in River Biofilms. SETAC Europe 32nd Annual Meeting, May 2022, Copenhagen, Denmark. ⟨hal-03960637⟩
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