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Poster De Conférence Année : 2021

Accumulation and effects of cobalt on river biofilms

Résumé

Cobalt (Co) is an essential metal involved as co-factor in several important biomolecules. Nevertheless, high concentrations can also be detrimental for biota and the increase of its environmental concentrations in specific water bodies may become an issue. The aim of the present study is thus to improve our knowledge on the short- and long-term effects of Co on microorganisms living in biofilms. To that end, two approaches were conducted. In the first set of experiments, biofilms were exposed to increasing Co concentrations (between 1.10-7 M and 1.10-5 M) using outdoor mesocosms (15 L) filled with the Gave de Pau river water. Biofilms were collected along with water at several sampling times (1, 3 and 7 days). In the second set of experiments, biofilms were exposed to 1.10-7 M, 5.10-7 M and 10-6 M Co in outdoor open-stream mesocosms filled with the Gave de Pau for 28 days. Biofilms and water were collected over time (1 hour, 1, 3, 7, 14, 21, 28 day). Water samples were analysed for the concentrations of cations (including Co), anions and dissolved organic carbon. Biofilms were examined for their total and intracellular Co concentrations. Effect of Co on biofilms is assessed by measuring modification of the biofilm chlorophyll-a content, of its community composition using targeted gene sequencing whereas untargeted metabolomic analyses are also performed. The obtained results will help us to better understand Co impacts on aquatic biota and to identify potential new biomarkers of Co exposure and effects.
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Dates et versions

hal-03960621 , version 1 (27-01-2023)

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  • HAL Id : hal-03960621 , version 1

Citer

Simon Colas, Mathieu Milhe-Poutingon, Patrick Baldoni-Andrey, Amiel Boullemant, Clémentine Gelber, et al.. Accumulation and effects of cobalt on river biofilms. 47th Canadian Ecotoxicity Workshop, Oct 2021, Halifax, Canada. ⟨hal-03960621⟩
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