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Communication Dans Un Congrès Année : 2021

Accumulation of cobalt in river biofilms

Résumé

Cobalt (Co) is an essential metal involved as co-factor in several important biomolecules such as the vitamin B12. Nevertheless, high concentrations can also detrimental for the biota. Anthropogenic activities are currently increasing its environmental concentrations. For instance, Co is extensively used in lithium-Co battery present in electrical appliances or electric cars. As with most contaminants, the aquatic compartment becomes the sink of released Co, exposing living organisms such as biofilms to these changes in concentration. However, little is known about the accumulation and toxicity of Co on biofilms. That lack of knowledge is impeding us to accurately protect inland waters. The aim of the present study is thus to improve our knowledge on the short- and long-term effects of Co on microbial communities. To that end, two approaches were conducted. In the first set of experiments, biofilms were exposed to increasing Co concentrations (1.10-7, 1.10-6, 5.10-6 and 1.10-5 M) using outdoor mesocosms (15 L) filled with the Gave de Pau water. Biofilms were collected along water at several sampling times (1, 3 and 7 days). In the second set of experiments, biofilms were exposed to 1.10-7, 5.10-7 and 10-6 M Co in outdoor open-stream mesocosms filled with the Gave de Pau for 28 days. Once again, biofims and water were collected at several step times (1 hour, 1, 3, 7, 14, 21, 28 day). Chemical and biological examination of those samples remained to be performed. Water will be analysed for the concentrations of cations (including Co), anions and dissolved organic carbon. Biofilms will be examined for their total and intracellular Co concentrations after an EDTA-washing step and acid digestion. Co effect will be assessed by measuring modification of the biofilm chlorophyll-a content, of its community composition using targeted gene sequencing whereas untargeted metabolomic analyses will also be performed. The obtained results would help us to better understand Co impacts on natural biofilm communities upon short and long-term exposure. Moreover, the assessment of several biological response will allow us to characterise potential new biomarkers of exposure and/or effects.
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Dates et versions

hal-03960611 , version 1 (27-01-2023)

Identifiants

  • HAL Id : hal-03960611 , version 1

Citer

Simon Colas, Mathieu Milhe-Poutingon, Patrick Baldoni-Andrey, Amiel Boullemant, Clémentine Gelber, et al.. Accumulation of cobalt in river biofilms. EcotoxicoMicYR 2021, EcotoxicoMic, Nov 2021, Virtual conference, France. ⟨hal-03960611⟩
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