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Communication Dans Un Congrès Année : 2022

A Metabolomic Approach to Determine the Effects of Cobalt on River Biofilms

Résumé

In the context of an energy transition, the mining of cobalt (Co) has been intensive over the past decade. As a consequence, an increase of its concentrations is observed in several aquatic environments, including rivers. The objective of this study is thus to examine Co effects on biofilms using untargeted metabolomics. For this purpose, mature biofilms colonized on glass slides were exposed to increasing Co concentrations (0, 10-7, 10-6, 5.10-6, 10-5 mol.L-1) for 7 days in triplicate in outdoor microcosms (15 L) filled with water from the river Gave de Pau. Each day, water was sampled whereas biofilms were collected after 1, 3 and 7 days of exposure in order to analyze for total and intracellular Co, chlorophyll-a content, community structure and metabolomic. Accumulated Co was mainly intracellular, and very good correlations between log10 concentrations of dissolved Co and intracellular Co concentrations were found at each exposure time (R2adj: 0.91, 0.95, 0.97 for days 1, 3 and 7, respectively). In total, 2117 metabolites were measured, and 159 were annotated. An important “microcosm” effect was noticed when comparing metabolites before and after exposure to microcosm conditions. An increase in lysodiacylglyceryltrimethyl homoserines (LDGTS), sphingosines and a decrease in flavonoids were observed in response to the increasing Co concentrations. An increase of LDGTS and sphingosines was also observed as a function of exposure time, as well as a decrease of lactones. Further biofilm analyses will determine the role of the biofilm composition on this modification of the metabolite composition.
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Dates et versions

hal-03960414 , version 1 (27-01-2023)

Identifiants

  • HAL Id : hal-03960414 , version 1

Citer

Simon Colas, B Marie, Mathieu Milhe-Poutingon, Patrick Baldoni-Andrey, Amiel Boullemant, et al.. A Metabolomic Approach to Determine the Effects of Cobalt on River Biofilms. 7th Aquatic Biofilm Workshop, Jun 2022, Bordeaux, France. ⟨hal-03960414⟩
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