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Poster De Conférence Année : 2018

Determination of glyphosate and AMPA in fish bile from the Marne River, France

Résumé

Glyphosate is a widely used herbicide. In France, it is used during intercultural operations and to control weeds in non-agricultural areas. Glyphosate and AMPA (its main degradation product) can reach high concentrations in rivers, especially in the Seine basin including the Paris suburb (France). Although it is not bioaccumulative, the associated use of surfactants in pesticide formulations allows better assimilation in biological tissues. The aim of our study was first to develop a method to assess glyphosate and AMPA levels in biological tissues and then to determine the contamination of an freshwater fish, the European chub (Squalius cephalus) by glyphosate and AMPA. Fish were fished in the Marne River (a tributary of the Seine River situated in the East part of Paris) at 4 sites characteristic of agricultural and urban areas. Water was also sampled for analysis to compare sites contamination. Bile is an ideal material to identify metabolites of pollutants. This biological fluid was taken directly from the gallbladder with a syringe on freshly euthanized chub and frozen for further analysis. Then, 100μL of bile was taken and 500μL of internal standard (13C-Glyphosate and 13C-AMPA) were added before extraction with milliQ water (5mL) and ultrasonic method for 30 minutes. The extract was then derivatizated (FMOC-Cl) and concentrated on SPE OASIS HLB cartridge (60cc) before LC MS MS analysis. Preliminary tests were performed to establish and validate the protocol and to find the lowest limit of quantification and the best reproducibility. Results showed that glyphosate is detected in a fish sample coming from the most contaminated site by AMPA. This suggests that glyphosate is assimilated in fish and is still detectable after glyphosate has been degraded to AMPA in the water river. Glyphosate content in fish could be an indicator of environmental contamination. Further developments are needed to validate the protocol and complete the study with other organs than bile.
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Dates et versions

hal-03948013 , version 1 (19-01-2023)

Identifiants

  • HAL Id : hal-03948013 , version 1

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Helene Blanchoud, Ferreux Teo, Fabrice Alliot, Aurélie Goutte. Determination of glyphosate and AMPA in fish bile from the Marne River, France. SETAC EUROPE, May 2018, ROME, Italy. , SETAC Europe 28th Annual Meeting Abstract Book, pp.309, 2018. ⟨hal-03948013⟩
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