Median adhesion forces of tritiated tungsten micro-particles deposited on a glass substrate were successfully determined using an aerodynamic method (AM) which is presented in this paper. The original aerodynamic device built for these experiments has been carefully characterized in terms of friction velocities allowing to quantify aerodynamic torque exerted on the particles and to deduce median adhesion forces thanks to a force balance approach. Using the same particle/surface systems (non-radioactive tungsten particles in contact with a glass substrate), distribution of adhesion forces were obtained using AFM for comparison with the AM. The results show a good agreement between the two techniques which allowed to validate the AM. Furthermore, a precise description of the root-mean square roughness (rms) distribution of the glass substrate made it possible to compare the experimental results with different analytical adhesion force models. Integrating the rms roughness distribution of the substrate into the model of Rabinovich et al. showed the best agreement with the present experiments capturing most of the adhesion forces of 10 mu m to 18 mu m diameter tungsten particles. Moreover, the method developed in this work made it possible to show that the electrostatic image force arising from the self-charging of tritiated tungsten particles has a negligible contribution in the adhesion of the particles for the studied configuration.