Assessment of a new technique combining a viability test, whole-cell hybridization and laser-scanning cytometry for the direct counting of viable Enterobacteriaceae cells in drinking water - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue FEMS Microbiology Letters Année : 2005

Assessment of a new technique combining a viability test, whole-cell hybridization and laser-scanning cytometry for the direct counting of viable Enterobacteriaceae cells in drinking water

Résumé

A new direct approach, called direct viable count (DVC)-FISH-ScanRDI, combining viability measurement, specific detection and sensitive enumeration of highly diluted Enterobacteriaceae cells, was assessed during the summer in water samples from a North American drinking water treatment plant and its distribution system. Major results of this field investigation show a higher sensitivity of the DVC-FISH-ScanRDI approach in enumerating viable Enterobacteriaceae cells in distributed drinking water, relative to a culture-based method, and the increased concentration of viable but non-culturable (VBNC) Enterobacteriaceae cells in distributed water for temperatures above 18 °C.
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hal-03941923 , version 1 (16-01-2023)

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Julia Baudart, Amandine Olaizola, Vincent Gauthier, Patrick Laurent, Josée Coallier. Assessment of a new technique combining a viability test, whole-cell hybridization and laser-scanning cytometry for the direct counting of viable Enterobacteriaceae cells in drinking water. FEMS Microbiology Letters, 2005, 243 (2), pp.405 - 409. ⟨10.1016/j.femsle.2005.01.001⟩. ⟨hal-03941923⟩
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