Compared with Milk Protein, a Wheat and Pea Protein Blend Reduces High-Fat, High-Sucrose Induced Metabolic Dysregulations while Similarly Supporting Tissue Protein Anabolism in Rats - Archive ouverte HAL Access content directly
Journal Articles Journal of Nutrition Year : 2023

Compared with Milk Protein, a Wheat and Pea Protein Blend Reduces High-Fat, High-Sucrose Induced Metabolic Dysregulations while Similarly Supporting Tissue Protein Anabolism in Rats

Didier Remond
Jérémie David
  • Function : Author
  • PersonId : 1174075
Nathalie Poupin

Abstract

Background: Plant proteins (PP) have been associated with better cardiovascular health than animal proteins (AP) in epidemiological studies. However, the underlying metabolic mechanisms remain mostly unknown. Objective: Using a combination of cutting-edge isotopic methods, we aimed at better characterizing the differences in protein and energy metabolisms induced by dietary protein sources (PP vs AP), in a prudent or western dietary context. Methods: Male Wistar rats (n=44, 8 weeks old) were fed for 4.5 months with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat), and being normal (NSF) or high (HFS) in sucrose (6 or 15%kcal, respectively) and saturated fat (7 or 20%kcal, respectively). We measured body weight and composition, hepatic enzyme activities and lipid content, and plasma metabolites. In the intestine, liver, adipose tissues and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis (DNL) using a 2H labelling method. Data were analyzed using ANOVA and mixed models. Results: At the whole-body level, PP limited HFS-induced insulin resistance (-27% in HOMA-IR between HFS groups, P<0.05). In the liver, PP induced lower lipid content (-17%, P<0.01) and DNL (-24%, P<0.05). In the different tissues studied, PP induced higher AA transamination accompanied by higher routings of dietary carbohydrate and lipid towards dispensable AA synthesis by glycolysis and β-oxidation, resulting in similar tissue PS and protein mass.
Fichier principal
Vignette du fichier
Lépine et al_2022_version acceptée_auteurs.pdf (719.25 Ko) Télécharger le fichier
Origin : Files produced by the author(s)
Licence : CC BY NC ND - Attribution - NonCommercial - NoDerivatives

Dates and versions

hal-03940315 , version 1 (12-05-2023)

Identifiers

Cite

Gaïa Lépine, Jean-François Huneau, Didier Remond, Véronique Mathé, Jérémie David, et al.. Compared with Milk Protein, a Wheat and Pea Protein Blend Reduces High-Fat, High-Sucrose Induced Metabolic Dysregulations while Similarly Supporting Tissue Protein Anabolism in Rats. Journal of Nutrition, 2023, 153 (3), pp.645-656. ⟨10.1016/j.tjnut.2022.12.029⟩. ⟨hal-03940315⟩
105 View
67 Download

Altmetric

Share

Gmail Facebook X LinkedIn More