INVESTIGATION OF ELECTROMAGNETIC FIELDS ON CARDIAC CELLS
Résumé
Exposure to electromagnetic fields (EMF) has become a sensitive topic for the public health. Despite accumulative exposure of humans to EMF, their effects on oxidative stress and cell death status in cardiac cells have not been studied. In the present study, the properties of transverse electromagnetic (TEM) cells were explored by varying the frequency and the power at first, then the temporal shape of the source EMF in a second time. In addition, we evaluated the effects of EMF on antioxidant and cell death/survival status of H9C2 cardiomyoblasts. Using reverse transcription quantitative PCR (RT-qPCR), we analyzed mRNA expression of antioxidant enzyme catalase, anti-apoptotic factor Bcl-2 and pro-apoptotic factor Bax in H9C2 cells subjected to 915 MHz for 24h, 48h and 72h. The exposure of H9C2 cells to EMF for 24h, 48h and 72h did not significantly change mRNA expression levels of catalase, Bcl-2 and Bax. Therefore, these results indicate that short-term exposure to EMF did not influence antioxidant and cell death status incardiomyoblasts.
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