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Article Dans Une Revue Blood Advances Année : 2022

RNA sequencing of chronic GVHD skin lesions defines shared and unique inflammatory pathways characterizing lichen planus and morphea

Habib Zouali
  • Fonction : Auteur
Juliette Lemasson
  • Fonction : Auteur
Andreea Calugareanu
Christophe Battail
David Michonneau
Chloé Grolleau
  • Fonction : Auteur
Charles Cassius
Marie Robin
Marine Merandet
Gabor Dobos
  • Fonction : Auteur
Thibault Mahevas
  • Fonction : Auteur
Michel Rybojad
  • Fonction : Auteur
Adèle de Masson
Reyhan Amode
  • Fonction : Auteur
Anne Boland
Laurence Michel
Flore Sicre de Fontbrune
Régis Peffault de Latour
  • Fonction : Auteur
Patrick Bruneval
Hafid Ait-Oufella
  • Fonction : Auteur
Maxime Battistella
Marie Jachiet
  • Fonction : Auteur
Martine Bagot
  • Fonction : Auteur
Jean-François Deleuze
  • Fonction : Auteur
Gérard Socié
  • Fonction : Auteur
Jean-David Bouaziz
  • Fonction : Auteur

Résumé

Abstract Cutaneous involvement of chronic graft-versus-host disease (cGVHD) has a wide range of manifestations including a lichenoid form with a currently assumed mixed Th1/Th17 signature and a sclerotic form with Th1 signature. Despite substantial heterogeneity of innate and adaptive immune cells recruited to the skin and of the different clinical manifestations, treatment depends mainly on the severity of the skin involvement and relies on systemic, high-dose glucocorticoids alone or in combination with a calcineurin inhibitor. We performed the first study using RNA sequencing to profile and compare the transcriptome of lichen planus cGVHD (n = 8), morphea cGVHD (n = 5), and healthy controls (n = 6). Our findings revealed shared and unique inflammatory pathways to each cGVHD subtype that are both pathogenic and targetable. In particular, the deregulation of IFN signaling pathway was strongly associated with cutaneous cGVHD, whereas the triggering receptor expressed on myeloid cells 1 pathway was found to be specific of lichen planus and likely contributes to its pathogenesis. The results were confirmed at a protein level by performing immunohistochemistry staining and at a transcriptomic level using real-time quantitative polymerase chain reaction.
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Dates et versions

hal-03873986 , version 1 (28-11-2022)

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Habib Zouali, Juliette Lemasson, Andreea Calugareanu, Christophe Battail, David Michonneau, et al.. RNA sequencing of chronic GVHD skin lesions defines shared and unique inflammatory pathways characterizing lichen planus and morphea. Blood Advances, 2022, 6 (9), pp.2805-2811. ⟨10.1182/bloodadvances.2021004707⟩. ⟨hal-03873986⟩

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