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Pré-Publication, Document De Travail Année : 2022

Actin-driven protrusions generate rapid long-range membrane tension propagation in cells

Résumé

Membrane tension is thought to be a long-range integrator of cell physiology. This role necessitates effective tension transmission across the cell. However, the field remains strongly divided as to whether cell membranes support or resist tension propagation, in part due to a lack of adequate tools for locally manipulating membrane tension. We overcome these limitations by leveraging optogenetics to generate localized actinbased protrusions while concurrently monitoring the propagation of membrane tension using dual-trap optical tweezers. Surprisingly, actin-driven protrusions elicit rapid global membrane tension propagation with little to no attenuation, while forces applied to the cell membrane only do not. We present a simple unifying mechanical model in which mechanical forces that act on both the membrane and actin cortex drive rapid, robust membrane tension propagation. Summary Mechanical perturbations acting on both actin cortex and plasma membrane drive global membrane tension increase within seconds

Dates et versions

hal-03866762 , version 1 (22-11-2022)

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Henry de Belly, Shannon Yan, Hudson Borja da Rocha, Sacha Ichbiah, Jason Town, et al.. Actin-driven protrusions generate rapid long-range membrane tension propagation in cells. 2022. ⟨hal-03866762⟩
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