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Article Dans Une Revue International Journal of Molecular Sciences Année : 2022

Synovial Extracellular Vesicles: Structure and Role in Synovial Fluid Tribological Performances

Layth Ben-Trad
Constantin Ionut Matei
  • Fonction : Auteur
Mirela Maria Sava
  • Fonction : Auteur
Samira Filali
  • Fonction : Auteur
Marie-Eve Duclos
  • Fonction : Auteur
Yves Berthier
  • Fonction : Auteur
Michel Guichardant
  • Fonction : Auteur
Nathalie Bernoud-Hubac
Ofelia Maniti
  • Fonction : Auteur
Ahmed Landoulsi
  • Fonction : Auteur
Marie-Genevieve Blanchin
  • Fonction : Auteur
Pierre Miossec
Thierry Granjon
  • Fonction : Auteur

Résumé

The quality of the lubricant between cartilaginous joint surfaces impacts the joint’s mechanistic properties. In this study, we define the biochemical, ultrastructural, and tribological signatures of synovial fluids (SF) from patients with degenerative (osteoarthritis-OA) or inflammatory (rheumatoid arthritis-RA) joint pathologies in comparison with SF from healthy subjects. Phospholipid (PL) concentration in SF increased in pathological contexts, but the proportion PL relative to the overall lipids decreased. Subtle changes in PL chain composition were attributed to the inflammatory state. Transmission electron microscopy showed the occurrence of large multilamellar synovial extracellular vesicles (EV) filled with glycoprotein gel in healthy subjects. Synovial extracellular vesicle structure was altered in SF from OA and RA patients. RA samples systematically showed lower viscosity than healthy samples under a hydrodynamic lubricating regimen whereas OA samples showed higher viscosity. In turn, under a boundary regimen, cartilage surfaces in both pathological situations showed high wear and friction coefficients. Thus, we found a difference in the biochemical, tribological, and ultrastructural properties of synovial fluid in healthy people and patients with osteoarthritis and arthritis of the joints, and that large, multilamellar vesicles are essential for good boundary lubrication by ensuring a ball-bearing effect and limiting the destruction of lipid layers at the cartilage surface.

Dates et versions

hal-03859927 , version 1 (18-11-2022)

Identifiants

Citer

Layth Ben-Trad, Constantin Ionut Matei, Mirela Maria Sava, Samira Filali, Marie-Eve Duclos, et al.. Synovial Extracellular Vesicles: Structure and Role in Synovial Fluid Tribological Performances. International Journal of Molecular Sciences, 2022, 23 (19), pp.11998. ⟨10.3390/ijms231911998⟩. ⟨hal-03859927⟩
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