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Article Dans Une Revue International Journal of Molecular Sciences Année : 2022

Comparative Analysis of Exo- and Endonuclease Activities of APE1-like Enzymes

Anastasiia Davletgildeeva
  • Fonction : Auteur
Alexandra Kuznetsova
  • Fonction : Auteur
Darya Novopashina
Alexander Ishchenko
Murat Saparbaev
Olga Fedorova
Nikita Kuznetsov

Résumé

Apurinic/apyrimidinic (AP)-endonucleases are multifunctional enzymes that are required for cell viability. AP-endonucleases incise DNA 5′ to an AP-site; can recognize and process some damaged nucleosides; and possess 3′-phosphodiesterase, 3′-phosphatase, and endoribonuclease activities. To elucidate the mechanism of substrate cleavage in detail, we analyzed the effect of mono- and divalent metal ions on the exo- and endonuclease activities of four homologous APE1-like endonucleases (from an insect (Rrp1), amphibian (xAPE1), fish (zAPE1), and from humans (hAPE1)). It was found that the enzymes had similar patterns of dependence on metal ions’ concentrations in terms of AP-endonuclease activity, suggesting that the main biological function (AP-site cleavage) was highly conserved among evolutionarily distant species. The efficiency of the 3′-5′ exonuclease activity was the highest in hAPE1 among these enzymes. In contrast, the endoribonuclease activity of the enzymes could be ranked as hAPE1 ≈ zAPE1 ≤ xAPE1 ≤ Rrp1. Taken together, the results revealed that the tested enzymes differed significantly in their capacity for substrate cleavage, even though the most important catalytic and substrate-binding amino acid residues were conserved. It can be concluded that substrate specificity and cleavage efficiency were controlled by factors external to the catalytic site, e.g., the N-terminal domain of these enzymes.
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Dates et versions

hal-03859362 , version 1 (24-11-2022)

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Anastasiia Davletgildeeva, Alexandra Kuznetsova, Darya Novopashina, Alexander Ishchenko, Murat Saparbaev, et al.. Comparative Analysis of Exo- and Endonuclease Activities of APE1-like Enzymes. International Journal of Molecular Sciences, 2022, 23 (5), pp.2869. ⟨10.3390/ijms23052869⟩. ⟨hal-03859362⟩
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