How Does Our Knowledge on the Na+/H+ Exchanger NHE1 Obtained by Biochemical and Molecular Analyses Keep up With Its Recent Structure Determination? - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Frontiers in Physiology Année : 2022

How Does Our Knowledge on the Na+/H+ Exchanger NHE1 Obtained by Biochemical and Molecular Analyses Keep up With Its Recent Structure Determination?

Résumé

Na + /H + exchangers are membrane transporters conserved in all living systems and therefore are assumed to be amongst the most ancestral molecular devices that equipped the first protocells. Following the cloning and sequencing of its gene, the mammalian NHE1, that regulates pH and volume in all cells, has been thoroughly scrutinized by molecular and biochemical analyses. Those gave a series of crucial clues concerning its topology, dimeric organization, pharmacological profile, regulation, and the role of key amino acids. Recently thanks to cryogenic Electron Microscopy (Cryo-EM) the long-awaited molecular structures have been revealed. With this information in mind we will challenge the robustness of the earlier conclusions and highlight how the new information enriches our understanding of this key cellular player. At the mechanistic level, we will pinpoint how the NHE1 3D structures reveal that the previously identified amino acids and regions are organized to coordinate transported cations, and shape the allosteric transition that makes NHE1 able to sense intracellular pH and be regulated by signaling pathways.
Fichier principal
Vignette du fichier
fphys-13-907587.pdf (2.78 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03855339 , version 1 (17-11-2022)

Identifiants

Citer

Mallorie Poet, Denis Doyen, Emmanuel van Obberghen, Gisèle Jarretou, Yann Bouret, et al.. How Does Our Knowledge on the Na+/H+ Exchanger NHE1 Obtained by Biochemical and Molecular Analyses Keep up With Its Recent Structure Determination?. Frontiers in Physiology, 2022, 13, ⟨10.3389/fphys.2022.907587⟩. ⟨hal-03855339⟩
18 Consultations
10 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More