BIN1/Amphiphysin 2 and ezrin drive filopodia-like structures in myoblasts - Archive ouverte HAL Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2022

BIN1/Amphiphysin 2 and ezrin drive filopodia-like structures in myoblasts

Franck Comunale
  • Fonction : Auteur
Charlotte André-Arpin
  • Fonction : Auteur
Hugo Bousquet
  • Fonction : Auteur
Feng-Ching Tsai
Félix Rico
Paolo Maiuri
  • Fonction : Auteur
Julien Pernier
  • Fonction : Auteur
Stéphane Bodin
  • Fonction : Auteur
Anne-Sophie Nicot
  • Fonction : Auteur
Jocelyn Laporte
  • Fonction : Auteur
Patricia Bassereau
Bruno Goud
Cécile Gauthier-Rouvière
  • Fonction : Auteur
Stéphanie Miserey

Résumé

Abstract Amphiphysin 2 (BIN1) is a membrane and actin remodeling protein mutated both in congenital and adult centronuclear myopathies. The BIN1 muscle-specific isoform finely tunes muscle regeneration in adulthood and regulates myoblast fusion. However, the underlying molecular mechanisms are unknown. Here, we report that BIN1 is required for myoblast fusion and participates in the formation of filopodia-like structures at myoblast intercellular junctions. BIN1 bundles actin in vitro and regulates the membrane-to-cortex attachment, two key processes required for myoblast fusion. We identified ezrin, a member of the ERM protein family, as a new BIN1 partner and showed that BIN1 promotes ezrin association to PI(4,5)P 2 at the cell cortex. Our results establish BIN1 and ezrin as central players at the early stages of myoblast fusion to form long-lived filopodia-like structures.

Dates et versions

hal-03854016 , version 1 (15-11-2022)

Identifiants

Citer

Laura Picas, Franck Comunale, Charlotte André-Arpin, Hugo Bousquet, Feng-Ching Tsai, et al.. BIN1/Amphiphysin 2 and ezrin drive filopodia-like structures in myoblasts. 2022. ⟨hal-03854016⟩
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