SECM for Studying the Immobilization and Repartition of a Redox Anti‐tetracycline Aptamer on Screen‐printed Carbon Electrodes
Résumé
Scanning electrochemical microscopy (SECM) is discussed as a versatile tool to provide a unique approach of localized electrochemical information in the context of biosensing research. The step-by-step immobilization of DNA aptamer with intrinsic redox activity on screen-printed carbon electrode (SPCE) was successfully monitored using SECM imaging tool. Control experiments were performed with a non-electroactive aptamer. After immobilization of these aptamers, SECM images showed the repartition of the electroactive anti-tetracycline aptamer when comparing with images produced for control and for all modification steps of SPCE. The possibility of tetracycline detection was also proved by causing a decrease in recorded current.
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