NtcA is responsible for accumulation of the small isoform of ferredoxin:NADP oxidoreductase - Archive ouverte HAL Access content directly
Journal Articles Microbiology Year : 2014

NtcA is responsible for accumulation of the small isoform of ferredoxin:NADP oxidoreductase

Amin Omairi-Nasser
  • Function : Author
Carla Galmozzi
  • Function : Author
Amel Latifi
  • Function : Author
M. Isabel Muro-Pastor
  • Function : Author

Abstract

In several cyanobacteria, petH , the gene encoding ferredoxin:NADP oxidoreductase (FNR), is transcribed from at least two promoters depending on growth conditions. Two transcripts (short and long) are translated from two different translation initiation sites, resulting in two isoforms (large and small, respectively). Here, we show that in Synechocystis PCC6803 the global transcriptional regulator NtcA activates transcription from the distal petH promoter. Modification of the NtcA-binding site prevents NtcA binding to the promoter in vitro and abolishes accumulation of the small isoform of FNR in vivo . We also demonstrate that a similar petH transcription and translation regime occurs in other cyanobacteria. The conditions under which this system operates provide hints for the function of each FNR isoform.

Domains

Bacteriology

Dates and versions

hal-03850156 , version 1 (12-11-2022)

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Cite

Amin Omairi-Nasser, Carla Galmozzi, Amel Latifi, M. Isabel Muro-Pastor, Ghada Ajlani. NtcA is responsible for accumulation of the small isoform of ferredoxin:NADP oxidoreductase: FNR isoform regulation in cyanobacteria.. Microbiology, 2014, 160 (4), pp.789-794. ⟨10.1099/mic.0.076042-0⟩. ⟨hal-03850156⟩
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