Plasmodium falciparum histidine rich protein 2 (pfhrp2): an additional genetic marker suitable for anti-malarial drug efficacy trials - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Malaria Journal Année : 2022

Plasmodium falciparum histidine rich protein 2 (pfhrp2): an additional genetic marker suitable for anti-malarial drug efficacy trials

Résumé

Background: Genotyping of the three Plasmodium falciparum polymorphic genes, msp1, msp2 and glurp, has been adopted as a standard strategy to distinguish recrudescence from new infection in drug efficacy clinical trials. However, the suitability of a particular gene is compromised in areas where its allelic variants distribution is significantly skewed, a phenomenon that might occur in isolated parasite populations or in areas of very low transmission. Moreover, observation of amplification bias has diminished the value of glurp as a marker. Methods: The suitability of the polymorphic P. falciparum histidine-rich protein 2 (pfhrp2) gene was assessed to serve as an alternative marker using a PCR-sequencing or a PCR–RFLP protocol for genotyping of samples in drug efficacy clinical trials. The value of pfhrp2 was validated by side-by-side analyses of 5 admission-recrudescence sample pairs from Yemeni malaria patients. Results: The outcome of the single pfhrp2 gene discrimination analysis has been found consistent with msp1, msp2 and glurp pool genotyping analysis for the differentiation of recrudescence from new infection. Conclusion: The findings suggest that under the appropriate circumstances, pfhrp2 can serve as an additional molecular marker for monitoring anti-malarials efficacy. However, its use is restricted to endemic areas where only a minority of P. falciparum parasites lack the pfhrp2 gene.
Fichier principal
Vignette du fichier
2022 MJ 21-2 Atroosh.pdf (1.84 Mo) Télécharger le fichier
2022 MJ 21-2 Atroosh Sup.xlsx (41.92 Ko) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03841078 , version 1 (06-11-2022)

Identifiants

Citer

Wahib M Atroosh, Yee-Ling Lau, Georges Snounou, Meram Azzani, Hesham M Al-Mekhlafi. Plasmodium falciparum histidine rich protein 2 (pfhrp2): an additional genetic marker suitable for anti-malarial drug efficacy trials. Malaria Journal, 2022, 21 (1), pp.2. ⟨10.1186/s12936-021-04014-4⟩. ⟨hal-03841078⟩
19 Consultations
19 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More