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Article Dans Une Revue Journal of Inorganic Biochemistry Année : 2015

The effect of phosphate on the nuclease activity of vanadium compounds

Nataliya Butenko
  • Fonction : Auteur
José Paulo da Silva
  • Fonction : Auteur
Ana Isabel Tomaz
  • Fonction : Auteur
João Costa Pessoa
  • Fonction : Auteur
Isabel Cavaco
  • Fonction : Auteur

Résumé

The nuclease activity of VO(acac)2 (1, acac = acetylacetone) and its derivatives VO(hd)2 (2, hd = 3,5- heptanedione), VO(Cl-acac)2 (3, Cl-acac = 3-chloro-2,4-pentanedione), VO(Et-acac)2 (4, Et-acac = 3-ethyl-2,4- pentanedione) and VO(Me-acac)2 (5, Me-acac = 3-methyl-2,4-pentanedione), is studied by agarose gel electropho- resis, UV–visible spectroscopy, cyclic and square wave voltammetry and 51V NMR. The mechanism is shown to be oxidative and associated with the formation of reactive oxygen species (ROS). Hydrolytic cleavage of the phospho- diester bond is also promoted by 1, but at much slower rate which cannot compete with the oxidative mechanism. The generation of ROS is much higher in the presence of phosphate buffer when compared with organic buffers and this was attributed to the formation of a mixed-ligand complex containing phosphate, (VIVO)(VVO)(acac)2(HnPO4n-3), presenting a quasi-reversible voltammetric behavior. The formation of this species was further observed by Electrospray Ionization Mass Spectrometry (ESI-MS). Phosphate being an essential species in most biological media, the importance of the formation of mixed-ligand species in other vanadium systems is emphasized.
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Dates et versions

hal-03839052 , version 1 (04-11-2022)

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Citer

Nataliya Butenko, José Paulo Pinheiro, José Paulo da Silva, Ana Isabel Tomaz, Isabel Correia, et al.. The effect of phosphate on the nuclease activity of vanadium compounds. Journal of Inorganic Biochemistry, 2015, 147, pp.165-176. ⟨10.1016/j.jinorgbio.2015.04.010⟩. ⟨hal-03839052⟩
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