c-Krox down-regulates the expression of UDP–glucose dehydrogenase in chondrocytes - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biochemical and Biophysical Research Communications Année : 2005

c-Krox down-regulates the expression of UDP–glucose dehydrogenase in chondrocytes

Résumé

Chondrocyte glycosaminoglycan (GAG) synthesis is regulated by the availability of UDP–glucuronate, the substrate of glucuronosyl transferases which form the GAG chains in proteoglycans and hyaluronan. UDP–glucose dehydrogenase (UDPGD) is therefore a key enzyme in the synthesis of UDP–glucuronate from glucose. However, the mechanisms regulating its expression in chondrocytes are not fully understood. We investigated the effect of c-Krox, a zinc-finger transcription factor previously shown to modulate several matrix genes, on the synthesis of GAG and transcriptional activity of several UDPGD gene promoter constructs, using transient transfection and decoy experiments in rabbit articular chondrocytes (RACs). We show that overexpression of c-Krox inhibits radiosulfate incorporation into neosynthesized GAG and that the effect was mediated by a cis-sequence located between +18 and +39 bp of the UDPGD gene. Since that sequence can also bind Sp1/Sp3 factors, it is likely that c-Krox acts in concert with these proteins to modulate the UDPGD gene expression in articular chondrocytes.

Dates et versions

hal-03838499 , version 1 (03-11-2022)

Identifiants

Citer

Gallic Beauchef, Magdalini Kypriotou, Christos Chadjichristos, Russell Widom, Benoît Porée, et al.. c-Krox down-regulates the expression of UDP–glucose dehydrogenase in chondrocytes. Biochemical and Biophysical Research Communications, 2005, 333 (4), pp.1123-1131. ⟨10.1016/j.bbrc.2005.06.020⟩. ⟨hal-03838499⟩
10 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More