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Article Dans Une Revue Nature Communications Année : 2022

Biased signaling due to oligomerization of the G protein-coupled platelet-activating factor receptor

Résumé

G protein-coupled receptors (GPCRs) are important drug targets that mediate various signaling pathways by activating G proteins and engaging β-arrestin proteins. Despite its importance for the development of therapeutics with fewer side effects, the underlying mechanism that controls the balance between these signaling modes of GPCRs remains largely unclear. Here, we show that assembly into dimers and oligomers can largely influence the signaling mode of the platelet-activating factor receptor (PAFR). Single-particle analysis results show that PAFR can form oligomers at low densities through two possible dimer interfaces. Stabilization of PAFR oligomers through cross-linking increases G protein activity, and decreases β-arrestin recruitment and agonist-induced internalization significantly. Reciprocally, β-arrestin prevents PAFR oligomerization. Our results highlight a mechanism involved in the control of receptor signaling, and thereby provide important insights into the relationship between GPCR oligomerization and downstream signaling.
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Origine : Publication financée par une institution

Dates et versions

hal-03837051 , version 1 (07-11-2022)

Identifiants

Citer

Junke Liu, Hengmin Tang, Chanjuan Xu, Shengnan Zhou, Xunying Zhu, et al.. Biased signaling due to oligomerization of the G protein-coupled platelet-activating factor receptor. Nature Communications, 2022, 13 (1), pp.6365. ⟨10.1038/s41467-022-34056-4⟩. ⟨hal-03837051⟩
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