The multivalent G-quadruplex (G4)-ligands MultiTASQs allow for versatile click chemistry-based investigations - Archive ouverte HAL Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2022

The multivalent G-quadruplex (G4)-ligands MultiTASQs allow for versatile click chemistry-based investigations

Francesco Rota Sperti
  • Fonction : Auteur
Jeremie Mitteaux
Joanna Zell
  • Fonction : Auteur
Angelique Pipier
Ibai Valverde

Résumé

Chemical biology hinges on multivalent molecular tools that uniquely allow for interrogating and/or manipulating cellular circuitries from the inside. The success of many of these approaches relies on molecular tools that make it possible to visualize biological targets in cells and then isolate them for identification purposes. To this end, click chemistry has become in just a few years a vital tool in offering practically convenient solutions to address highly complicated biological question. We report here on two clickable molecular tools, the biomimetic G-quadruplex (G4) ligands MultiTASQ and azMultiTASQ, which benefit from the versatility of two types of bioorthogonal chemistry, CuAAC and SPAAC (the discovery of which was very recently awarded the Nobel Prize of chemistry). These two MultiTASQs are here used to both visualize G4s in, and identify G4s from human cells. To this end, we developed click chemo-precipitation of G-quadruplexes (G4-click-CP) and in situ G4 click imaging protocols, which provide unique insights into G4 biology in a straightforward and reliable manner.

Domaines

Chimie
Fichier principal
Vignette du fichier
Rota Sperti et al - MultiTASQ.pdf (1.74 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03836044 , version 1 (04-11-2022)

Identifiants

Citer

Francesco Rota Sperti, Jeremie Mitteaux, Joanna Zell, Angelique Pipier, Ibai Valverde, et al.. The multivalent G-quadruplex (G4)-ligands MultiTASQs allow for versatile click chemistry-based investigations. 2022. ⟨hal-03836044⟩
32 Consultations
31 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More