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Article Dans Une Revue Polymer Année : 2022

Enzymatically-Active Nanoparticles to Direct the Self-Assembly of Peptides in Hydrogel With a 3D Spatial Control

Résumé

Hydroxypropylmethylcellulose grafted with silanol groups (Si-HPMC) hydrogels are employed as 3D scaffolds to encapsulate phosphatase-modified silica nanoparticles (AP@NPs). Such hybrid materials are catalytically active when enzyme susbtrates are diffusing through the material. Using the enzyme-assisted self-assembly concept, the tripeptide Fmoc-FFY is generated in situ in close vicinity of AP@NPs thanks to the phosphate hydrolysis of the diffusing precursor Fmoc-FFpY. The self-assembly of Fmoc-FFY is spatially concomitant to the presence of AP@NPs. In the Si-HPMC host hydrogel, AP@NPs aggregate and thus limit their diffusion in the material. This feature is used to localize at the micrometer scale the self-assembly within the material: AP@NPs are injected in a defined 3D area within the Si-HPMC gel using an adapted microsyringe. The diffusion of precursor Fmoc-FFpY toward this region leads to a Fmoc-FFY self-assembly exclusively there, an impossible strategy using free AP. Thus, this work allows to spatially control the enzyme-assisted self-assembly of peptides within host materials as an alternative approach for the design of hierarchically structured materials.
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Dates et versions

hal-03832303 , version 1 (27-10-2022)

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Citer

Miryam Criado-Gonzalez, Jean-Yves Runser, Alain Carvalho, Fouzia Boulmedais, Pierre Weiss, et al.. Enzymatically-Active Nanoparticles to Direct the Self-Assembly of Peptides in Hydrogel With a 3D Spatial Control. Polymer, 2022, 261, pp.125398. ⟨10.1016/j.polymer.2022.125398⟩. ⟨hal-03832303⟩
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