Does apelin interact with GnRH and kisspeptin neuronal populations to modulate the central control of reproduction in male rodents? A neuroanatomical, molecular and functional investigation - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2022

Does apelin interact with GnRH and kisspeptin neuronal populations to modulate the central control of reproduction in male rodents? A neuroanatomical, molecular and functional investigation

Résumé

BACKGROUND: Hypothalamic control of reproduction relies on GnRH and kisspeptin (KP) secretions. KP neurons are sensitive to sex steroids and metabolic status and their distribution overlaps with neurons producing apelin, a metabolic hormone known to decrease LH secretion in rodents. Previous reports showed an acute effect of apelin on LH secretion in male rats. Our aim here was to unravel the mechanisms induced by apelin occurring at the hypothalamic level and more specifically at the level of KP neurons from the arcuate nucleus and at the level of GnRH neurons distributed more rostrally. RESULTS: Using double immunohistochemical staining and confocal microscopy, we first observed neuroanatomical contacts between apelin fibers and both KP and GnRH neurons in the hypothalamus of male rats and mice suggesting a possible direct action of apelin on the activities of these two populations. We then performed intracerebroventricular apelin infusion for 2 weeks in male mice using Alzet pumps. Chronic apelin treatment did not decrease LH levels nor did it affect gene expression for KP, neurokinin B and dynorphin. Finally, a third experiment was conducted on GnRH neurons cultured from nasal explants. It showed that increasing apelin concentrations did not modulate Ca2+ levels of cultured GnRH neurons, while 10µM apelin infusion on forskolin pretreated GnRH neurons reevoked a rhythmic activity in 18% of GnRH neurons. SUMMARY: These results suggest that acute apelin effect on LH secretion does not involve modulation of gene expression in KP neurons but may affect the secretory activity of GnRH neurons.
Fichier non déposé

Dates et versions

hal-03832244 , version 1 (28-10-2022)

Identifiants

  • HAL Id : hal-03832244 , version 1

Citer

Anne Abot, Vincent Robert, Renaud Fleurot, Hugues Dardente, Vincent Hellier, et al.. Does apelin interact with GnRH and kisspeptin neuronal populations to modulate the central control of reproduction in male rodents? A neuroanatomical, molecular and functional investigation. Polish-French Symposium on new regulations of reproduction: the role of adipokines, Oct 2022, Paris, France. ⟨hal-03832244⟩
45 Consultations
2 Téléchargements

Partager

Gmail Facebook X LinkedIn More