Removal of a Membrane Anchor Reveals the Opposing Regulatory Functions of Vibrio cholerae Glucose-Specific Enzyme IIA in Biofilms and the Mammalian Intestine - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue mBio Année : 2018

Removal of a Membrane Anchor Reveals the Opposing Regulatory Functions of Vibrio cholerae Glucose-Specific Enzyme IIA in Biofilms and the Mammalian Intestine

Vidhya Vijayakumar
  • Fonction : Auteur
Audrey Vanhove
Bradley Pickering
  • Fonction : Auteur
Julie Liao
  • Fonction : Auteur
Braden Tierney
  • Fonction : Auteur
John Asara
  • Fonction : Auteur
Roderick Bronson
  • Fonction : Auteur
Paula Watnick

Résumé

The V. cholerae phosphoenolpyruvate phosphotransferase system (PTS) is a well-conserved, multicomponent phosphotransfer cascade that regulates cellular physiology and virulence in response to nutritional signals. Glucose-specific enzyme IIA (EIIA Glc ), a component of the PTS, is a master regulator that coordinates bacterial metabolism, nutrient uptake, and behavior by direct interactions with protein partners. We show that an amphipathic helix (AH) at the N terminus of V. cholerae EIIA Glc serves as a membrane association domain that is dispensable for interactions with cytoplasmic partners but essential for regulation of integral membrane protein partners. By removing this amphipathic helix, hidden, opposing roles for cytoplasmic partners of EIIA Glc in both biofilm formation and metabolism within the mammalian intestine are revealed. This study defines a novel paradigm for AH function in integrating opposing regulatory functions in the cytoplasm and at the bacterial cell membrane and highlights the PTS as a target for metabolic modulation of the intestinal environment.

Dates et versions

hal-03826790 , version 1 (24-10-2022)

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Vidhya Vijayakumar, Audrey Vanhove, Bradley Pickering, Julie Liao, Braden Tierney, et al.. Removal of a Membrane Anchor Reveals the Opposing Regulatory Functions of Vibrio cholerae Glucose-Specific Enzyme IIA in Biofilms and the Mammalian Intestine. mBio, 2018, 9 (5), ⟨10.1128/mBio.00858-18⟩. ⟨hal-03826790⟩
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