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Article Dans Une Revue Applied and Environmental Microbiology Année : 2020

Methionine Availability in the Arthropod Intestine Is Elucidated through Identification of Vibrio cholerae Methionine Acquisition Systems

Audrey Vanhove
Bat-Erdene Jugder
  • Fonction : Auteur
Daniela Barraza
  • Fonction : Auteur
Paula Watnick

Résumé

Methionine is an essential amino acid involved in both biosynthetic and regulatory processes in the bacterial cell. To ensure an adequate supply of methionine, bacteria have evolved multiple systems to synthesize, import, and recover this amino acid. To explore the importance of methionine synthesis, transport, and recovery in any environment, all of these systems must be identified and mutagenized. Here, we have mutagenized every high-affinity methionine uptake system and methionine sulfoxide reductase encoded in the genome of the diarrheal pathogen V. cholerae . We use this information to determine that high-affinity methionine uptake systems are sufficient to acquire methionine in the intestine of the model arthropod Drosophila melanogaster but are not involved in virulence and that the intestinal concentration of methionine must be between 0.05 mM and 0.5 mM.

Dates et versions

hal-03826788 , version 1 (24-10-2022)

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Citer

Audrey Vanhove, Bat-Erdene Jugder, Daniela Barraza, Paula Watnick. Methionine Availability in the Arthropod Intestine Is Elucidated through Identification of Vibrio cholerae Methionine Acquisition Systems. Applied and Environmental Microbiology, 2020, 86 (11), ⟨10.1128/AEM.00371-20⟩. ⟨hal-03826788⟩
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