Actuating tension-loaded DNA clamps drives membrane tubulation - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Science Advances Année : 2022

Actuating tension-loaded DNA clamps drives membrane tubulation

Longfei Liu
Qiancheng Xiong
Chun Xie
Frederic Pincet
Chenxiang Lin

Résumé

Membrane dynamics in living organisms can arise from proteins adhering to, assembling on, and exerting force on cell membranes. Programmable synthetic materials, such as self-assembled DNA nanostructures, offer the capability to drive membrane-remodeling events that resemble protein-mediated dynamics but with user-defined outcomes. An illustrative example is the tubular deformation of liposomes by DNA nanostructures with purposely designed shapes, surface modifications, and self-assembling properties. However, stimulus-responsive membrane tubulation mediated by DNA reconfiguration remains challenging. Here, we present the triggered formation of membrane tubes in response to specific DNA signals that actuate membrane-bound DNA clamps from an open state to various predefined closed states, releasing prestored energy to activate membrane deformation. We show that the timing and efficiency of vesicle tubulation, as well as the membrane tube widths, are modulated by the conformational change of DNA clamps, marking a solid step toward spatiotemporal control of membrane dynamics in an artificial system.
Fichier principal
Vignette du fichier
2022.05.02.490361v1.full.pdf (8.55 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03825409 , version 1 (25-10-2022)

Identifiants

Citer

Longfei Liu, Qiancheng Xiong, Chun Xie, Frederic Pincet, Chenxiang Lin. Actuating tension-loaded DNA clamps drives membrane tubulation. Science Advances , 2022, 8 (41), ⟨10.1126/sciadv.add1830⟩. ⟨hal-03825409⟩
23 Consultations
24 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More