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Single molecule imaging simulations with full fluorophore photophysics

Dominique Bourgeois
  • Function : Correspondent author

Abstract

Advanced fluorescence imaging techniques such as single-molecule localization microscopy (SMLM) fundamentally rely on the photophysical behavior of the employed fluorophores. This behavior is generally complex and impacts data quality in a subtle manner. A simulation software named SMIS (Single-Molecule Imaging Simulator) is introduced that simulates a widefield microscope and incorporates fluorophores with arbitrarily complex photophysical properties. With SMIS, intricate data collection schemes combining 3D, multicolor, single-particle-tracking or quantitative SMLM can be implemented virtually and the influence of imaging and environmental parameters evaluated.
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hal-03810049 , version 1 (11-10-2022)

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Dominique Bourgeois. Single molecule imaging simulations with full fluorophore photophysics. 2022. ⟨hal-03810049⟩
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