The LH-DH module of the bacterial replicative helicases is the common binding site for DciA and other helicase loaders
Résumé
During the initiation step of bacterial genome replication, replicative helicases depend on specialized proteins for their loading onto oriC . DnaC and DnaI were the first loaders characterized. However, most bacteria do not contain any of these genes, which are domesticated phage elements that replaced the ancestral and unrelated loader gene dciA several times during evolution. To understand how DciA assists the loading of DnaB, we determined the crystal structure of the complex from Vibrio cholerae , in which two Vc DciAs interact with a dimer of Vc DnaB, without changing its canonical structure. Our data showed that the Vc DciA binding site on Vc DnaB is the conserved module formed by the linker helix LH of one monomer and the determinant helix DH of the second one. Interestingly, DnaC from Escherichia coli also targets this module onto Ec DnaB. Thanks to their common target site, we showed that Vc DciA and Ec DnaC could be functionally interchanged in vitro , despite sharing no structural similarities. This is a milestone in understanding the mechanism employed by phage helicase loaders to hijack bacterial replicative helicases during evolution.
Domaines
Biologie structurale [q-bio.BM]
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