The apo ‐form of the Vibrio cholerae replicative helicase DnaB is a labile and inactive planar trimer of dimers
Résumé
To enable chromosomal replication, DNA is unwound by the ATPase molecular motor replicative helicase. The bacterial helicase DnaB is a ring-shaped homo-hexamer whose conformational dynamics are being studied through its different 3D structural states adopted along its functional cycle. Our findings describe a new crystal structure for the apo-DnaB from Vibrio cholerae, forming a planar hexamer with pseudo-symmetry, constituted by a trimer of dimers in which the C-terminal domains delimit a triskelion-shaped hole. This hexamer is labile and inactive. We suggest that it represents an intermediate state allowing the formation of the active NTP-bound hexamer from dimers.
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FEBS Letters - 2022 - Cargemel - The apo‐form of the Vibrio cholerae replicative helicase DnaB is a labile and inactive.pdf (2 Mo)
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