The apo ‐form of the Vibrio cholerae replicative helicase DnaB is a labile and inactive planar trimer of dimers - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue FEBS Letters Année : 2022

The apo ‐form of the Vibrio cholerae replicative helicase DnaB is a labile and inactive planar trimer of dimers

Résumé

To enable chromosomal replication, DNA is unwound by the ATPase molecular motor replicative helicase. The bacterial helicase DnaB is a ring-shaped homo-hexamer whose conformational dynamics are being studied through its different 3D structural states adopted along its functional cycle. Our findings describe a new crystal structure for the apo-DnaB from Vibrio cholerae, forming a planar hexamer with pseudo-symmetry, constituted by a trimer of dimers in which the C-terminal domains delimit a triskelion-shaped hole. This hexamer is labile and inactive. We suggest that it represents an intermediate state allowing the formation of the active NTP-bound hexamer from dimers.
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Dates et versions

hal-03808118 , version 1 (10-10-2022)

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Claire Cargemel, Hélène Walbott, Dominique Durand, Pierre Legrand, Malika Ouldali, et al.. The apo ‐form of the Vibrio cholerae replicative helicase DnaB is a labile and inactive planar trimer of dimers. FEBS Letters, 2022, 596 (16), pp.2031-2040. ⟨10.1002/1873-3468.14403⟩. ⟨hal-03808118⟩
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